Differences in pulmonary group 2 innate lymphoid cells are dependent on mouse age, sex and strain

Journal article

Loering S, Cameron GJM, Bhatt NP, Belz GT, Foster PS, Hansbro PM, Starkey MR.
Immunol Cell Biol, vol. 99(5), 2020, pp. 542-551

DOI: 10.1111/imcb.12430

Full text PubMed

Cite

APA Click to copy
S, L., GJM, C., NP, B., GT, B., PS, F., PM, H., & MR., S. (2020). Differences in pulmonary group 2 innate lymphoid cells are dependent on mouse age, sex and strain. Immunol Cell Biol, 99(5), 542–551. https://doi.org/10.1111/imcb.12430

Chicago/Turabian Click to copy
S, Loering, Cameron GJM, Bhatt NP, Belz GT, Foster PS, Hansbro PM, and Starkey MR. “Differences in Pulmonary Group 2 Innate Lymphoid Cells Are Dependent on Mouse Age, Sex and Strain.” Immunol Cell Biol 99, no. 5 (2020): 542–551.

MLA Click to copy
S, Loering, et al. “Differences in Pulmonary Group 2 Innate Lymphoid Cells Are Dependent on Mouse Age, Sex and Strain.” Immunol Cell Biol, vol. 99, no. 5, 2020, pp. 542–51, doi:10.1111/imcb.12430.

BibTeX Click to copy

@article{loering2020a,
  title = {Differences in pulmonary group 2 innate lymphoid cells are dependent on mouse age, sex and strain},
  year = {2020},
  issue = {5},
  journal = {Immunol Cell Biol},
  pages = {542-551},
  volume = {99},
  doi = {10.1111/imcb.12430},
  author = {S, Loering and GJM, Cameron and NP, Bhatt and GT, Belz and PS, Foster and PM, Hansbro and MR., Starkey}
}

Abstract

Innate lymphoid cells (ILCs) are resident in the lung and are involved in both the maintenance of homeostasis and the pathogenesis of respiratory diseases. In this study, murine lung ILCs were characterized using flow cytometry and the impact of mouse age, sex and strain were assessed. Lung ILCs were found as early as postnatal day 4 and numbers peaked at 2 weeks, and then decreased as the lung matured. During postnatal lung development, ILC expressed differential amounts of group 2 ILC (ILC2)-associated cell surface antigens including ST2, CD90.2 and ICOS. Using Il5venus Il13td-tomato dual reporter mice, neonates were found to have increased constitutive interleukin (IL)-13 expression compared with adult mice. Neonates and adults had similar ratios of IL-5+ CD45+ leukocytes; however, these cells were mostly composed of ILCs in neonates and T cells in adults. Sex-specific differences in ILC numbers were also observed, with females having greater numbers of lung ILCs than males in both neonatal and adult mice. Female lung ILCs also expressed higher levels of ICOS and decreased KLRG1. Mouse strain also impacted on lung ILCs with BALB/c mice having more ILCs in the lung and increased expression of ST2 and ICOS compared with C57BL/6J mice. Collectively, these data show that lung ILC numbers, cell surface antigen expression, IL-5 and IL-13 levels differed between neonatal and adult lung ILCs. In addition, cell surface antigens commonly used for ILC2 quantification, such as ST2, CD90.2 and ICOS, differ depending on age, sex and strain and these are important considerations for consistent universal identification of lung ILC2s.

Keywords: group 2 innate lymphoid cells (ILC2); infant; innate lymphoid cells (ILC); lung; neonate; respiratory.

https://doi.org/10.1111/imcb.12430